Bulk pollen pollination in maize for efficient construction of introgression populations with high genome coverage

Introgression populations consist of a set of introgression lines or families, constructed by continuous backcrossing to the recurrent parent, while carrying a limited number of chromosome segments from a donor parent in their genomes. Increasing the genome coverage is an important aim when constructing introgression population. In this study, we proposed bulk pollen pollination (BPP) method and used it to increase the genome coverage of a maize introgression population. The results showed that the genome coverage of the introgression population constructed using BPP method reached 100% at BC₃ generation, which accorded with the simulation result. The BPP‐based BC₃F_1:2 population could identify most quantitative trait loci (QTL) detected using the F_2:3 population, especially major QTL. Simulation analysis showed that the genome coverage of introgression population increased with the increase of population size and the number of bulked plants, and decreased with the increase of backcross generation. Our results proved the reliability of the BPP‐based introgression population in increasing genome coverage and detecting QTL, and provided references for constructing high‐coverage introgression populations.     

我国牧草种植成本收益变化与比较

本研究以牧草种植户牧草及粮食作物的调研数据为基础,计算出2010-2014年主要牧草和粮食作物的成本收益基本信息,对其变化进行统计分析,并对各作物的成本收益进行比较。分析发现,2014年苜蓿(Medicago sativa)的经济效益有所下降;黑麦草(Lolium perenne)和青贮玉米(Zea mays)的纯收益和收益率有所增长;苜蓿的纯收益和收益率要高于黑麦草和青贮玉米;马铃薯(Solanum tuberosum)和苜蓿的经济效益更具优势,种植小麦(Triticum aestivum)经济效益较差;黑麦草和青贮玉米人工和土地成本更高,苜蓿水电费最高。建议调整农业产业结构,推行"粮+经+饲+草"四元种植结构,全面扶持牧草产业发展,建立健全牧草生产资料补贴制度。     

马铃薯AP2/ERF转录因子的克隆及植物表达载体构建

AP2/ERF基因家族转录因子普遍存在于植物中,参与植物体内的各种生物学过程,包括植物的生长发育、生物和非生物胁迫响应等。前期转录组测序的结果发现马铃薯‘加湘1号’一个AP2/ERF家族基因(PGSC0003DMG400012154)在接种晚疫病菌(Phytophthora infestans)24 h后被显著激活。从接种P.infestans 24 h的‘加湘1号’的总RNA中通过RT-PCR获得了该基因CDS序列为885 bp,BLAST分析显示该基因编码一个295个氨基酸残基的蛋白,并且含有1个AP2/ERF结构域,是AP2/ERF转录因子家族中的ERF亚家族的一员。本研究将该基因与XcmⅠ酶切的表达载体pCXSN连接,转化大肠杆菌,通过测序挑选插入正确克隆酶切验证,并成功转化农杆菌。本研究结果为进一步研究该基因的功能提供了帮助。     

株行距及穴苗数的配置对寒地水稻产量和品质的影响

为明确垦稻26和龙粳31高产优质栽培的株行距和穴苗数处理,采取裂区设计进行试验研究。结果表明,最优群体因品种而异,垦稻26在株距10cm、行距30cm、5苗/穴产量最佳,龙粳31则以株距13.3cm、行距27cm、9苗/穴产量最佳;垦稻26在5苗/穴、行株距30cm×10cm处理,有利于提高稻米的碾磨品质,不利于改善稻米的外观品质,营养品质较低,利于改善稻米的食味品质,且食味评分值高达86.5,显著高于最低处理;龙粳31在5苗/穴、行株距27cm×13.3cm处理,碾磨品质较高,不利于营养品质和外观品质的改善,食味品质得到明显改善,食味评分值高达86.0,与最低处理差异达显著水平。因此,高产优质栽培要因品种选择适宜的穴苗数和株行距,是实现水稻高产优质最为快捷、最为经济有效的措施。     

Leaf carbohydrates assimilation and metabolism affect seed yield of rapeseed with different waterlogging tolerance under the interactive effects of nitrogen and waterlogging

Waterlogging is a main factor causing rapeseed (Brassica napus L.) yield loss, and reasonable nitrogen (N) applications can compensate for this loss. To investigate the effects of N rates on seed yield of waterlogged rapeseed, the waterlogging-tolerant rapeseed variety ZS 9 and sensitive variety GH01 were waterlogged for 0 and 10 days with five leaves at the seedling stage under four N rates (0, 90, 180 and 270 kg/ha). Waterlogging significantly decreased seed yield, while N application can alleviate the yield loss. The yield decrease rate of waterlogged GH01 was greater than that of ZS 9 under the same N rate. During the seedling and bolting stage, the leaf photosynthetic rate (Pn) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity increased, while activities of adenosine diphosphate glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) decreased with more N under the same watering conditions. Compared to the plants without waterlogging, the leaf Pn and Rubisco activity, starch and sucrose contents of waterlogged rapeseed decreased at the two stages; activities of AGPase, SuSy and SPS of waterlogged rapeseed decreased at the seedling while increased at the bolting stage for both the two varieties. At the flowering stage, the Pn, the activities of Rubisco, AGPase, SuSy, SPS and contents of sucrose, starch increased with more N application for both ZS 9 and GH01. Compared to the plants without waterlogging, the Pn and Rubisco activity for waterlogged plants of the two varieties increased; the waterlogged plants of tolerant variety had higher activities of AGPase, SuSy and SPS, while those of sensitive variety was significantly lower. However, the decreased starch and sucrose content were found in both tolerant and sensitive varieties. The activities of AGPase, SuSy and SPS at flowering were highly positively correlated with yield under the interactive effects of N and waterlogging. These results suggested that the flowering stage is the most important stage that N had the positive regulation on waterlogged rapeseed growth. The carbohydrates translocation from leaves to seeds of the tolerant variety were enhanced after waterlogging, while that of the sensitive variety was still inhibited. This was the main reason for the difference in yield between the two waterlogged varieties.     

IWOS系统在配网故障处理中的应用

随着我国经济社会的不断发展,全社会用电量不断上升,对电网的可靠性、安全性和电能质量也提出越来越高的要求。由于历史原因,配网建设欠账较多,总体基础薄弱,发展落后于主网,区域、城乡之间发展不均衡,水平差异大,存在明显短板。随着电动汽车、分布式电源、微电网、储能装置的快速发展,对配电网的安全性、经济性、互动性、适应性提出越来越高的要求。在国家电网公司建设“大运行”体系、推进地调县调一体化进程背景下,建设安全智能的配网自动化系统成为当务之急,但现有配网线路大多存在着技术、设备落后等问题,自动化水平不高,难以直接建成大规模的配网自动化系统。     

浙江省耕地多功能价值时空变化与权衡-协同关系

耕地多功能价值的时空演变与权衡-协同关系测度对于深化耕地可持续利用和管理具有重要意义。该研究以经济快速发展地区浙江省为例,采用价值量化方法对2000、2010和2015年浙江省耕地多功能进行价值评估并分析其时空变化特征。运用Spearman秩相关系数法、双变量空间自相关模型以及可拓展随机性环境影响评估(StochasticImpactsby Regression on Population,Affluence and Technology,STIRPAT)模型探究耕地多功能之间的权衡-协同关系及影响因素。结果表明:1)2000—2015年浙江省耕地多功能总价值下降,地均耕地多功能价值呈现先下降后上升的趋势。其中气体调节、水源涵养和社会保障功能价值下降明显,食物生产和美学景观功能价值增加;耕地多功能总价值在空间上呈现北高南低的分布格局,2000—2015年大部分县市耕地多功能总价值均有不同程度的下降,西南山地丘陵区耕地多功能总价值有所提升。2)浙江省耕地多功能之间主要表现为协同关系,各项功能之间的协同-权衡关系存在空间异质性;2000—2015年,浙江省耕地多功能协同关系总体减弱,在空间上主要表现为高值协同区减少。3)城镇居民可支配收入和地均农业机械总动力对耕地多功能总价值有负面影响,农村居民可支配收入增加有助于耕地多功能总价值的提升。该研究成果可为科学划定耕地利用与保护区,促进耕地多功能的协同利用和提升耕地资源价值提供科学依据。     

陈将三兄弟:迪尔青贮机让致富路越走越宽

1988年出生的陈将,是安徽省蚌埠市五河县农机大户,投身农机作业服务已有7年多了,算得上是"老农机"了。如今拥有5台约翰迪尔进口青贮饲料收获机,以及其他农机具产品,总资产1 000多万元。没见到陈将之前,很难想象这么年轻的一位小伙,竟有如此的实力和魄力,在青贮机械化收获事业上越做越大,成了远近闻名的农机大户,并且是迪尔公司的"铁粉"。     

中国林产工业产业关联与波及效应分析——基于投入产出模型的国际比较

文中基于中国、美国、俄罗斯、印度、巴西、印度尼西亚6国2000—2014年投入产出数据，通过测算中间需求率、中间投入率、感应度系数和影响力系数，对林产工业产业关联与波及效应进行动态比较分析。研究结果表明:相较于其他国家，中国木材加工业、造纸业的中间产品型产业特征明显，对国民经济贡献程度较高，但同时也面临成为“瓶颈产业”的风险；中国家具制造业的最终需求型产业性质显著，受整体经济发展的拉动作用明显弱于其他国家。总体而言，中国林产工业对国民经济的推动作用整体上高于国民经济发展对其的拉动作用。需要重点提升木材加工业、家具制造业整体产业链竞争力，加大科研创新投入力度以提升产出效率，并进一步提高家具制造业整体产品的附加值。